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The Antarctic ice sheet is the world's largest freshwater reservoir and the ice-free oceans surrounding it are densely populated by a broad variety of zooplankton, fish and other organisms, many of which share an intimate genetic relationship with the ice. Therefore, these organisms are valuable subjects for studying natural levels of DNA damage. DNA repair has already been examined in Antarctic fishes, but few studies have investigated DNA damage in Arctic fish. We investigated the effect of light intensity, temperature and acclimation period on DNA repair capacity of a phylogenetically diverse sample of Antarctic and Arctic fish. For this purpose, a total of eight different fish species from four different orders (Clupeiformes, Perciformes, Nototheniiformes and Perciformes), which were exposed to two contrasting light intensities (3.3 and 20.7 μmol photons m(-2) s(-1)), two different acclimation temperatures (0 and 10°C) and eight different periods of acclimation (0, 1, 2, 4, 8, 16, 30 and 60 days). The comet assay, a single cell electrophoresis technique, was used for assessment of DNA damage. The results showed that the response of fish species to the different light intensities, temperatures and periods of acclimation was highly variable, as species responded with very distinct DNA repair capacities. This variability among species indicates that studies investigating the effects of changes in natural environments on DNA repair capacity are needed for planning and implementing the necessary conservation measures in a given ecosystem.// Copyright 2014 The Go Authors. All rights reserved.

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